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Your Yourlocation: Home > Effects of tiotropium bromide(136310-93-5) on respiratory function and pathological changes in rats with chronic obstructive pulmonary disease

Objective: To investigate the effects of tiotropium bromide(136310-93-5) on the pathogenesis and respiratory function of chronic obstructive pulmonary disease(COPD).

Methods: Thirty rats were randomly divided into 3 groups, each group with 10. Blank control group(group A): normal feeding for 12 weeks; model group (group B): 1 day, 14 days and 29 days tracheal infusion of 200μg (200μL) saline lipopolysaccharide (LPS), smoked every day (Group C): The COPD model was established in the same manner as in the model group, and the rats were treated with atomized tiotropium (30 minutes before smoking) , 0.12 mmoL·L-1, 20 min, 60 nmol) for 12 weeks. BALF and serum interleukin-6 (IL-6) and leukotriene-4 (IL-6) were measured by HE staining in the right middle lobe lung tissue. The BALF was measured by ELISA. 

Results: ① Compared with the normal group, the body weight of the model group decreased significantly after 4 weeks (P <0.05). Compared with the model group, the weight gain was increased at 2 weeks after the intervention of tiotropium bromide(136310-93-5)(P <0.05 ). ② Compared with the blank control group, the airway resistance of the model group was significantly increased (P <0.01), lung compliance was significantly decreased (P <0.01), 0.2s expiratory volume (FEV0.2) / maximum vital capacity (FVC ) and respiratory peak velocity (PEF) decreased significantly (P <0.05). The airway resistance of tiotropium group was significantly lower than that of the model group (P <0.05), and the compliance was significantly higher than that in the blank control group (P <0.05). The compliance was significantly higher than that in the model group (P <0.05) FVC (%) decreased significantly (P <0.05), but there was no significant difference between the two groups (P <0.05). ③ The airway inflammation of the model group increased and the emphysema was obvious. The treatment group also appeared inflammation and emphysema, but the degree was mild. Compared with the blank control group, the total number of cells, monocytes, macrophages, neutrophils and lymphocytes were significantly increased in the model group (P <0.01). The number of cells, monocytes, macrophages and neutrophils in BALF group were significantly lower than those in model group (P <0.01). The levels of IL-8 and LTC4 in the serum and BALF of the model group were significantly higher than those in the blank control group (P <0.05). The levels of IL-6 and LTC4 in the treatment group were significantly lower than those in the model group (P <0.01).

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