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Your Yourlocation: Home > Quantification of Olodaterol and Tiotropium Bromide(136310-93-5) by High Performance Liquid Chromatography

This paper describes a sensitive, accurate and accurate RP-HPLC method for the simultaneous quantitation of olodaterol and tiotropium bromide(136310-93-5). Chromatography was performed on a Thermo Hypersil C8 analytical column (150 mm × 4.6 mm, 5 µ particle size). The isocratic elution system was developed using phosphate buffer: methanol [55:45 v/v]. The pH of the mobile phase was adjusted to 4.4 with orthophosphoric acid. The elution of the analytes was performed at a flow rate of 1 mL / min. Detection was performed by UV absorbance at a wavelength of 236 nm. For both analytes, the detector response was linear over the concentration range of 12.5-37.5 µg/mL. The limits of detection and limits of quantitation for olodaterol were found to be 0.037 and 0.124 12.5-37.5 µg/mL, respectively, and for tiotropium were 0.023 and 0.079 12.5-37.5 µg/mL, respectively. The method was validated according to the ICH guidelines. All of the validation parameters are within the acceptable range.

(1R) -1-hydroxy-2 - [[1- (4-methoxyphenyl) -2-methylpropan-2-yl] amino] oxetane, Ethyl] -4H-1,4-benzoxazin-3-one is an adrenergic beta-2 receptor agonist. Ototerol is highly selective for beta-2 receptors located in the lungs. Lt; / RTI & gt; and beta-3 adrenergic receptors, which are typically located in cardiac smooth muscle and adipose tissue, respectively. Lortolol binding to the beta-2 receptor leads to smooth muscle relaxation and bronchiectasis in the lungs. 

Odtero is prescribed for the management of symptoms of chronic obstructive pulmonary disease.Tiotropium bromide(136310-93-5), chemically described as (1α,2β,4β,7β)-7-[(hydroxidi-2-thienylacetyl)oxy]-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.02,4]nonane bromide, is a antimuscarinic agent or also referred as cholinergic antagonist. Tiotropium bromide mainly acts on M3 muscarinic receptors present in the smooth muscle cells, preventing smooth muscle contraction, as a result producing a bronchodilatory effect. The cholinergic antagonist effect of tiotropium bromide is to block the effect of cholinergic nerves, leading to the relaxation of muscles and dilation of airways. Tiotropium bromide is prescribed for the management of chronic obstructive pulmonary disease.

Olodaterol and tiotropium bromide combination is used in the treatment of airflow obstruction in patients with chronic obstructive pulmonary disease. The combination of these two drugs with different mechanisms of action improves breathing more than either drug alone. Tiotropium bromide and olodaterol combination inhalation spray (Stiolto Respimat, Boehringer Ingelheim Pharmaceuticals, Inc., USA), was approved by the Food and Drug Administration in 2015.

Literature survey revealed that no RP-HPLC method has been reported for simultaneous quantitation of olodaterol and tiotropium bromide(136310-93-5). Hence, a RP-HPLC method has been developed in the present work for simultaneous estimation of olodaterol and tiotropium bromide in the presence of their stress degradants.

Tiotropium bromide, chemically described as (1α, 2β, 4β, 7β) -7 - [(hydroxy-2-thienylacetyl) oxy] -9,9-dimethyl- Azabicyclo [3.3.1.1 2,4] bromide, is an antimuscarinic agent or also a cholinergic antagonist. Tiotropium bromide mainly acts on M3 muscarinic receptors present in smooth muscle cells, preventing smooth muscle contraction, resulting in bronchodilation. The role of tiotropium bromide as a cholinergic antagonist is to block the action of cholinergic nerves, leading to muscle relaxation and airway dilation. Tiotropium bromide for the treatment of chronic obstructive pulmonary disease.

Combination of otatroterone and tiotropium in the treatment of airflow obstruction in patients with chronic obstructive pulmonary disease. The combination of these two drugs with different mechanisms of action improves respiration more than either drug alone. (Stiolto Respimat, Boehringer Ingelheim Pharmaceuticals, Inc., USA), which was approved by the Food and Drug Administration in 2015.

No RP-HPLC method has been reported for simultaneous quantitation of olodaterol and tiotropium, according to literature surveys. Accordingly, RP-HPLC methods have been developed in this work for the simultaneous evaluation of olodaterol and tiotropium bromide(136310-93-5) in the presence of their stress degradation products.

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